Abcam, ab259234, Human USP42 knockout HeLa cell lysate

Size: 1Kit
USP42 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 2 and 5 bp deletion in exon 2 and 7 bp deletion in exon 2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 2 and 5 bp deletion in exon 2 and 7 bp deletion in exon 2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-USP42, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
USP42 also known as Ubiquitin-specific peptidase 42 is part of the ubiquitin-proteasome system. USP42 specifically removes ubiquitin molecules from specific protein substrates. This action involves deubiquitination a process that regulates protein degradation. The molecular weight of USP42 is approximately 150 kDa. In terms of expression USP42 is found in various tissues including the liver brain and kidneys indicating it has a broad role across different systems of the body.
Biological function summary
The function of USP42 is important in gene regulation and cell cycle control. By controlling protein stability it influences transcriptional activity and cellular responses to stress. USP42 associates with the p53 protein stabilizing it and enhancing its tumor suppressor function. Additionally it forms part of larger protein complexes that coordinate DNA damage responses. Through these interactions USP42 supports cellular health by ensuring proper repair and replication of DNA.
Pathways
In these pathways USP42 plays a part in modulating ubiquitin ligases and deubiquitinases activities maintaining protein homeostasis. The protein acts in concert with others like Mdm2 and p53 important for cellular stress responses and apoptosis. These pathways highlight the impact of USP42 in balancing cell survival and programmed cell death mechanisms.
Altered USP42 expression or function has links to tumorigenesis due to its interaction with proteins such as p53. Dysregulation in this pathway may contribute to cancer proliferation. In neurodegenerative conditions abnormal protein aggregation occurs if ubiquitin-proteasome function is impaired connecting to USP42's role in maintaining protein quality control. The protein's dysfunction can therefore influence disease progression by failing to regulate protein turnover properly.