Abcam, ab259242, Human WDR59 knockout HeLa cell lysate

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WDR59 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 47 bp deletion in exon1 and Insertion of the selection cassette in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 47 bp deletion in exon1 and Insertion of the selection cassette in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-WDR59, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
WDR59 also known as WD repeat domain 59 functions as a member of the WD repeat protein family. This protein weighs approximately 61 kDa. It plays a role as a part of the GATOR2 complex and regulates mTORC1 signaling. Researchers have observed its expression in various tissues including the brain and liver reflecting its broad functional importance in cellular activities.
Biological function summary
WDR59 interacts with various proteins and facilitates amino acid sensing by mTORC1. It forms part of a subcomplex known as GATOR2 which acts in opposition to GATOR1 to activate mTORC1 when cellular amino acid levels are sufficient. WDR59's involvement in amino acid signaling highlights its role in nutrient sensing pathways influencing cellular growth and metabolism.
Pathways
WDR59 affects mTOR signaling and the PI3K-Akt pathway. By participating in mTORC1 regulation it helps dictate cell growth proliferation and survival in response to nutrient availability. Its interaction with other GATOR2 components such as WDR24 and SEH1L illustrates its integration in nutrient sensing and metabolic regulation pathways.
Changes in WDR59 function associate with certain cancers especially where mTOR signaling malfunctions are part of the pathology. For instance errant mTOR pathway signaling may contribute to tumor growth. Additionally WDR59 might link to neurodevelopmental disorders considering its brain expression and roles in cellular signaling pathways. Interactions with proteins like DEPDC5 can highlight these associations as both are involved in mTOR pathway regulation.