Abcam, ab259410, Recombinant human TNF alpha protein (Active)

Size: 10µg / 50µg / 100µg / 250µg
Recombinant human TNF alpha protein (Active) is a Human Full Length protein in the 77 to 233 aa range with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for Functional studies, Cell Culture, ELISA, SDS-PAGE and more. The predicted molecular weight of ab259410 protein is 17.4 kDa. - Save time and ensure accurate results- use our TNF alpha protein as a control - Optimal protein bioactivity and stability - Available in different sizes to fit your experimental needs
Key facts
Purity:>95% SDS-PAGE,
Endotoxin level:< 0.005 EU/µg,
Expression system:HEK 293 cells,
Tags:Tag free,
Applications:Cell Culture, HPLC, sELISA, SDS-PAGE, FuncS, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant Killing/apoptosis of L-929 cells is 0.71ng/mL corresponding to a Specific Activity of 1.41 x 106 IU/mg.,
Accession:P01375,
Animal free:Yes,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute in PBS,
Storage buffer:pH: 6 - 8Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Product details:
Ensure the validity of your result using our recombinant human TNF alpha protein ab259410 as a control.
The ab259410 TNF alpha protein is sourced from HEK293 cells and can be used as a positive control in SDS-PAGE, mass spectrometry and HPLC. Analyze your TNF alpha ELISA data using the ab259410 protein to generate and plot a standard curve.
Check out our protein gel staining guide for SDS-PAGE
Check out our ELISA protocol for more information
Premium Bioactive Protein range
The ab259410 TNF alpha protein is part of the premium bioactive protein range which are ideal for preclinical cell culture and functional studies. These recombinant proteins are of the highest activity, purity, and consistency, meeting rigorous biophysical characterization.
More premium bioactive proteins can be found

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient