Abcam, ab25969, Anti-West Nile Virus (E protein) antibody

Size: 100µg
Rabbit Polyclonal POLG antibody. Suitable for ELISA and reacts with West Nile virus samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:West Nile virus,
Applications:ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The West Nile Virus E protein alternatively known as the envelope protein plays an important role in the viral life cycle. It exhibits a modular structure and functions as a glycoprotein on the virus's surface. The E protein facilitates viral entry into host cells by mediating membrane fusion a critical step for viral infection. With an approximate mass of 53 kDa this protein is embedded in the lipid envelope of the virus. It is expressed during the assembly and budding process of the virus ultimately becoming an integral part of the virion surface.
Biological function summary
The West Nile Virus E protein participates in essential functions like receptor binding and conformational changes required for initiating infection. This protein is a part of the viral envelope contributing to the formation of virion and its subsequent infectivity. The E protein undergoes structural rearrangements to facilitate the fusion of the viral envelope with the host cell membrane. This action involves forming fusion loops which are regions that directly interact with the target membrane.
Pathways
The membrane fusion activity of the West Nile Virus E protein is central to the viral entry pathway. This pathway includes interactions with host cell surface receptors and subsequent endocytosis. It closely relates to other flavivirus envelope proteins like those of dengue and Zika viruses sharing a mechanism of inducing fusion at low pH within endosomes. The E protein's interaction with cellular pathways allows it to manipulate host processes and ensures successful viral replication and spread.
The West Nile Virus E protein is directly associated with West Nile fever and neuroinvasive disease. Its role in viral entry and immune evasion contributes to disease pathogenesis. The E protein interacts with host proteins during infection helping the virus to escape immune response and establish infection. This interaction stresses the importance of targeting the E protein for therapeutic strategies and vaccine development. Understanding its role can lead to strategies to prevent the spread of related diseases like Japanese encephalitis which shares similar pathways and protein interactions.