Abcam, ab259986, Anti-TRMT5 antibody [EPR24139-11]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal TRMT5 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24139-11,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRMT5 also known as tRNA methyltransferase 5 is an enzyme responsible for catalyzing the methylation of N1 at position G37 of mitochondrial and cytoplasmic tRNA using S-adenosylmethionine as a methyl donor. This process is essential in maintaining proper tRNA structure and function. TRMT5 has a molecular mass of approximately 54 kDa and is expressed in a wide range of human tissues with more concentration in the liver and kidney.
Biological function summary
The role of TRMT5 is to facilitate correct tRNA folding and stability impacting translation accuracy. It does so by targeting the guanine base adjacent to the anticodon in tRNA molecules enhancing the fidelity of protein synthesis. While TRMT5 functions predominantly as a solitary enzyme its activity impacts other cellular processes by ensuring the efficiency of protein translation machinery.
Pathways
TRMT5 influences the tRNA modification pathway which is essential for correct and efficient protein translation. This enzyme collaborates with other proteins like TRMT6 that engage in further tRNA modifications within the process. Together they enhance the translation mechanisms that ripple into broader pathways such as the mitochondrial protein translation pathway necessary for cellular energy metabolism.
Researchers have implicated TRMT5 in certain mitochondrial dysfunctions. Dysregulation or mutations in TRMT5 can lead to altered tRNA stability contributing to systemic disorders like mitochondrial encephalomyopathy. Moreover TRMT5 is associated with neurological disorders that might parallel connections with proteins involved in mitochondrial translation fidelity such as GTPBP3 which safeguards against translational error.