Abcam, ab261854, Human SMARCC1 (BAF155) knockout HEK-293 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SMARCC1 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. Knockout achieved by CRISPR/Cas9; X = 1 bp insertion, 2 bp insertion, 1 bp insertion. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,Western blot,
Mutation description:Knockout achieved by CRISPR/Cas9; X = 1 bp insertion, 2 bp insertion, 1 bp insertion

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SMARCC1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SMARCC1 also known as BAF155 is a component of the SWI/SNF chromatin remodeling complex with a molecular weight of approximately 155 kDa. It plays a role in modifying the structure of chromatin which affects the accessibility of DNA to transcription factors and other proteins. SMARCC1 expresses widely in various tissues particularly where regulation of gene transcription is essential. This protein acts as a scaffold facilitating the assembly of the SWI/SNF complex which is vital for the regulation of transcriptional processes.
Biological function summary
SMARCC1 participates in remodeling chromatin architecture to facilitate or repress gene transcription. It forms an integral part of the SWI/SNF complex which is critical for maintaining proper chromatin structure and function. This complex influences gene expression during developmental processes cell cycle regulation and DNA repair. SMARCC1 interacts with other core components of the SWI/SNF complex such as BAF47 and BRG1 ensuring correct and responsive chromatin modification.
Pathways
SMARCC1 participates in the ATP-dependent chromatin remodeling pathway an important player in gene expression control. This remodeling is connected to the cell cycle pathway as it regulates genes required for cell proliferation. Within these pathways SMARCC1 closely interacts with transcription factors such as c-Myc and other chromatin regulators coordinating the expression of target genes necessary for cell growth and differentiation.
SMARCC1 is linked to cancer and neurodevelopmental disorders. Alterations in SMARCC1 function or expression can lead to dysregulated gene expression contributing to tumorigenesis by affecting pathways involved in cell growth and apoptosis. For instance its association with BRG1 influences pathways implicated in various cancers including solid tumors. Additionally SMARCC1 dysfunction connects to certain neurodevelopmental disorders through the modification of genes critical for brain development and function.