Abcam, ab263084, Human ANAPC15 (APC15) knockout HEK-293T cell lysate

Size: 1Kit
ANAPC15 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ANAPC15, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
APC15 also known as Anaphase Promoting Complex subunit 15 is an important regulatory component of the anaphase-promoting complex/cyclosome (APC/C). This protein with a molecular mass of around 16 kDa plays an important role in cell cycle regulation. APC15 expression occurs in various tissues where it contributes to the timely progression of the cell cycle by controlling the degradation of specific cell cycle regulators. It functions mechanistically by recognizing and targeting substrates for ubiquitination which marks them for degradation by the proteasome.
Biological function summary
APC15 contributes to the molecular machinery that drives cell cycle transitions. It is part of the larger APC/C complex a multi-subunit E3 ubiquitin ligase complex essential for maintaining the proper sequence of cell cycle events particularly during mitosis. APC15's regulatory activity ensures that cells do not prematurely enter anaphase enabling the correct chromosomal alignment and segregation. It interacts with other complex subunits to coordinate precise ubiquitination of proteins that inhibit chromosome separation until the cellular environment is ready.
Pathways
APC15 plays a significant role in cell cycle pathways particularly the cell cycle G2/M transition and mitotic exit. It works closely with other proteins like CDC20 and CDH1 which serve as co-activators to manage progression through the cell cycle. These interactions help direct the APC/C complex to its specific substrates at various stages ensuring fidelity in chromosome segregation and the prevention of aneuploidy a condition with too many or too few chromosomes.
APC15 has a connection to cancer where dysregulation can lead to abnormal cell division and tumorigenesis. This dysregulation often involves overexpression or malfunctioning of associated proteins such as CDC20 impacting the APC/C complex's ability to perform adequate protein degradation and cell cycle control. Additionally defects in the APC/C machinery which includes APC15 have been implicated in neurodegenerative diseases where aberrant protein accumulation and misregulation disrupt normal cellular function.