Abcam, ab263255, Human MPRIP knockout HeLa cell lysate

Size: 1Kit
MPRIP KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4 and Insertion of the selection cassette in exon4.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4 and Insertion of the selection cassette in exon4.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MPRIP, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MPRIP also known as Myosin Phosphatase Rho Interacting Protein or p116Rip is an important player in the regulation of the actin cytoskeleton. This protein weighs approximately 116 kDa and is expressed in a variety of tissues including the brain heart and testis. It interacts with the regulatory subunit of myosin phosphatase influencing actin filament dynamics by modulating the interaction between actin and myosin.
Biological function summary
MPRIP serves a critical role in the modulation of actin cytoskeleton rearrangement. It is part of a multi-protein complex that includes myosin phosphatase which is essential for dephosphorylation of myosin light chains. By binding directly to the myosin phosphatase complex MPRIP ensures proper regulation of actin-myosin interactions which are integral to cellular processes such as migration division and shape maintenance.
Pathways
MPRIP plays an important role within the RhoA signaling pathway and the regulation of cytoskeletal rearrangements. It facilitates the interaction between RhoA and myosin phosphatase leading to the deactivation of contractility. Another important protein involved is ROCK as MPRIP counteracts ROCK-induced phosphorylation of myosin light chain therefore influencing cell contractility and motility.
MPRIP dysregulation has implications in cardiovascular diseases and cancer. Its connection with proteins like ROCK and RhoA highlights its role in these pathologies. In cardiovascular diseases improper MPRIP function can lead to aberrant vascular smooth muscle contraction while in cancer altered MPRIP activity may contribute to changes in cell migration and invasion affecting cancer progression and metastasis.