Abcam, ab263323, Human QRICH1 (Glutamine-rich protein 1) knockout HeLa cell lysate

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QRICH1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon3 and Insertion of the selection cassette in exon3.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon3 and Insertion of the selection cassette in exon3.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-QRICH1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
QRICH1 also known as Glutamine-rich protein 1 is a protein that plays an essential role in transcriptional regulation. It has a molecular mass of approximately 91 kDa. This protein is highly expressed in human tissues such as the heart liver and brain. It functions as a transcription factor influencing the expression of genes by binding to specific DNA regions. QRICH1 contains a glutamine-rich region which is typical for transcription factors that assist in the regulation of gene expression.
Biological function summary
QRICH1 modulates cellular processes that involve gene expression. It is part of the transcriptional machinery complex interacting directly with other transcriptional regulators and co-activators. This interaction influences cellular activities such as proliferation differentiation and stress responses. QRICH1 regulates genes involved in cell cycle control and apoptosis affecting how cells respond to various internal and external signals. Its biological role makes it an important target for understanding gene regulatory networks.
Pathways
QRICH1 plays a role in pathways significant to cellular stress responses and apoptosis. It interacts with proteins such as p53 and E2F1 which are important in these pathways. QRICH1 helps mediate cellular responses to DNA damage working alongside p53 in the apoptotic signaling pathway. Additionally its interaction with transcription factors such as E2F1 highlights its involvement in cell cycle regulation and the balance between cell survival and apoptosis.
QRICH1 has connections to cancer and neurodegenerative diseases. In cancer alterations in QRICH1 expression or function can lead to uncontrolled cell proliferation due to its role in regulating genes tied to the cell cycle and apoptosis. Its interaction with p53 and E2F1 further points to its involvement in tumorigenesis when dysregulated. In neurodegenerative disorders QRICH1's expression in the brain tissue suggests it might play a role possibly connected to stress response pathways related to neurodegeneration. Understanding its function could uncover targets or markers for therapeutic interventions in these diseases.