Abcam, ab263334, Human RQCD1 knockout HeLa cell lysate

Size: 1Kit
CNOT9 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon1 and 1 bp insertion in exon1 and 68 bp deletion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon1 and 1 bp insertion in exon1 and 68 bp deletion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-CNOT9, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RQCD1 also known as Required for Cell Differentiation 1 is a protein with an approximate mass of 34 kDa. It plays an essential role in gene expression regulation by acting as a transcriptional cofactor. RQCD1 is highly expressed in various tissues including the liver kidney and heart. The protein interacts with different transcription factors and modulates their activities which influences cellular pathways critical for cell development and differentiation.
Biological function summary
The protein RQCD1 acts as a component of the human mRNA methyltransferase-associated complex. The complex is involved in post-transcriptional modifications. Through its interaction in the complex RQCD1 contributes to RNA processing and stability further affecting gene expression outcomes. This involvement impacts cell cycle regulation and proliferation necessary for maintaining cellular homeostasis.
Pathways
The protein RQCD1 is important in the MAPK signaling pathway and Wnt signaling pathway. In these pathways it interacts with proteins like c-Fos and β-catenin influencing pathways related to cell growth and differentiation. Its presence in these pathways demonstrates the protein's role in signal transduction processes that govern various cellular responses.
Proteins like RQCD1 have shown associations with cancer and metabolic disorders. Aberrant expression or mutations in RQCD1 are linked to the development and progression of certain cancers due to its role in transcriptional regulation. Additionally this protein has connections with metabolic proteins like AMPK indicating its potential impact on disorders related to metabolic imbalance. These roles suggest that RQCD1 is a significant player in both cancer biology and metabolism-related diseases.