Abcam, ab263337, Human RYK knockout HeLa cell lysate

Size: 1Kit
RYK KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 160 bp deletion in exon2 and 1 bp deletion in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 160 bp deletion in exon2 and 1 bp deletion in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RYK, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The receptor-like tyrosine kinase (RYK) also known as RYK receptor-like tyrosine kinase is a protein involved in Wnt signaling pathways. It has a molecular mass of approximately 74 kDa. RYK functions as a single-pass type I membrane protein and primarily acts as a receptor for Wnt proteins. It is expressed in various tissues including brain testis and pancreas reflecting its diverse roles in developmental and physiological processes.
Biological function summary
RYK modulates cellular communication by interacting with Wnt proteins. It does not possess intrinsic kinase activity but participates in signaling as part of a larger receptor complex on the cell surface. This complex includes other proteins such as Frizzled receptors and LRP co-receptors facilitating the translation of extracellular Wnt signals into intracellular responses. RYK although not a conventional kinase plays a pivotal role in neuronal development influencing axon guidance and synaptic formation.
Pathways
RYK is integral to the Wnt signaling pathway which is essential for cell proliferation differentiation and migration. Through the Wnt pathway RYK interacts with other proteins such as Disheveled (Dvl) to execute its signaling functions. Additionally RYK has associations with the non-canonical Wnt/PCP (planar cell polarity) pathway modulating tissue polarity and cell movement during embryogenesis. Its role within these pathways highlights the intricate regulatory networks involved in developmental biology.
RYK has associations with cancer and neurodevelopmental disorders. Aberrant expression or mutations in RYK can contribute to tumor growth and metastasis implicating it in cancers such as glioma. During neurodevelopment alterations in RYK signaling can lead to disorders like autism spectrum disorder (ASD). RYK interacts with proteins such as β-catenin in pathological states influencing the progression of these conditions. Understanding RYK's involvement in these diseases provides insights that could facilitate therapeutic strategies.