Abcam, ab263340, Human SCO1 knockout HEK-293T cell lysate

Size: 1Kit
SCO1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SCO1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
'SCO1' also known as SCO1 homolog is a protein involved in the assembly of cytochrome c oxidase the last enzyme in the mitochondrial respiratory chain. SCO1 has a mass of approximately 33 kDa and is commonly expressed in tissues with high energy demands such as muscle and brain tissue. The protein plays a mechanical role in copper delivery to cytochrome c oxidase subunits.
Biological function summary
SCO1 participates in the transfer of copper ions essential for the catalytic activity of cytochrome c oxidase. It is a component of the cytochrome c oxidase assembly complex. This function ensures that the enzyme maintains its activity which is necessary for efficient cellular respiration. SCO1 interacts with other mitochondrial proteins responsible for stabilizing complex formation and securing enzyme functionality.
Pathways
SCO1 contributes to the oxidative phosphorylation pathway and is important for the proper functioning of the mitochondrial electron transport chain. Its role in copper ion transfer is vital for energy production in cells. SCO1 interacts with the protein SCO2 another homolog involved in copper binding and transport to cytochrome c oxidase highlighting their interconnected tasks within this pathway.
Defects in SCO1 are linked to mitochondrial disorders specifically linked to cytochrome c oxidase deficiency and Leigh syndrome. These conditions involve disruptions in energy metabolism and present with severe clinical manifestations. Mutations in SCO1 can influence its interaction with other proteins such as COX17 essential for copper metabolism exacerbating the mitochondrial dysfunction in affected individuals.