Abcam, ab263344, Human SH2B2 (APS) knockout HEK-293T cell lysate

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SH2B2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and Insertion of the selection cassette in exon2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and Insertion of the selection cassette in exon2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SH2B2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
APS also known as SH2B adapter protein 2 acts as an adaptor in various signaling pathways. It is a protein with a mass of approximately 80 kDa. The APS is expressed in many tissues but largely found in the spleen and brain. It features an SH2 domain and plays a significant role in modulating signaling cascades.
Biological function summary
APS participates in cell signaling by linking activated receptors to downstream signaling proteins. It often associates with receptor tyrosine kinases such as the insulin receptor. APS can form part of a larger complex with these kinases influencing cellular processes like glucose lipid metabolism and growth. APS interactions are essential for transducing signals that affect metabolic and growth pathways.
Pathways
APS is notably involved in the insulin signaling pathway and the JAK-STAT pathway. In insulin signaling APS supports insulin receptor functions by promoting the recruitment of key proteins such as PI3K and Akt which are important for glucose uptake and lipid synthesis. In the JAK-STAT pathway APS acts by facilitating interactions with the JAK family of kinases which affects gene expression and immune responses.
APS associations connect it to metabolic conditions and cancer. Altered APS expression or function can affect insulin signaling contributing to insulin resistance and type 2 diabetes. In cancer abnormal APS interactions might play a role in signaling pathways that lead to tumor growth. APS links to proteins like IRS1 in diabetes which alters glucose metabolism and STAT proteins in cancer impacting cell proliferation and survival.