Abcam, ab263352, Human SLC30A7 (ZnT-7) knockout HeLa cell lysate

Size: 1Kit
SLC30A7 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 4 bp deletion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 4 bp deletion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC30A7, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ZnT-7 also known as SLC30A7 is a zinc transporter protein responsible for decreasing cytosolic zinc levels by transporting zinc ions into the Golgi apparatus and trans-Golgi network. The molecular weight of ZnT-7 is approximately 45 kDa. It is expressed in many tissues with high levels found in the liver kidney and heart. The protein is localized mainly in the intracellular membranes playing a role in intracellular zinc homeostasis.
Biological function summary
ZnT-7 participates in the regulation of cellular zinc balance and is important for maintaining optimal enzyme activity and protein function. Although it does not form part of a larger complex itself it interacts with other zinc transporters to help coordinate zinc distribution within cells. This management of zinc levels is important for preventing zinc toxicity and supporting normal cellular activities which are dependent on zinc as a cofactor.
Pathways
ZnT-7 is involved in the zinc homeostasis pathway controlling the intracellular sequestration and distribution of zinc. It works alongside other zinc transporters such as ZnT-1 and ZIP family members to maintain appropriate intracellular zinc levels. ZnT-7 also affects the insulin signaling pathway as zinc ions are modulators of insulin storage and secretion in pancreatic beta cells.
Alterations in ZnT-7 function or expression have been linked to type 2 diabetes and prostate cancer. Disruption of zinc homeostasis due to ZnT-7 malfunction can contribute to metabolic dysfunctions and tumor development. The protein’s interaction with other zinc transporters including ZnT-10 highlights its role in these conditions emphasizing the need for proper regulation of zinc levels in disease prevention and management.