Abcam, ab263393, Human TMEM69 knockout HeLa cell lysate

Size: 1Kit
TMEM69 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TMEM69, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The TMEM69 protein also referred to as Transmembrane Protein 69 has a mass of approximately 31 kDa. It acts as an integral membrane protein involved in lipid signaling and cellular communication. Expression of TMEM69 occurs in various tissues with notable presence in the brain and heart. Structurally its transmembrane domains facilitate interaction with other membrane proteins enabling effective signal transmission across the cell membrane.
Biological function summary
The functions associated with TMEM69 contribute to cellular signaling processes and membrane trafficking. The protein does not form part of larger protein complexes itself but interacts with several membrane-associated proteins for diverse cellular roles. Its activity impacts cellular homeostasis influencing both signal transduction pathways and the maintenance of cellular machinery.
Pathways
TMEM69 impacts both the PI3K/AKT signaling and ERK/MAPK pathways influencing cell growth and survival. In the PI3K/AKT pathway it enhances kinase activity contributing to cell survival and proliferation. Within the ERK/MAPK pathway TMEM69 modulates protein interactions leading to changes in cell differentiation. Proteins like AKT and ERK1/2 interact closely with TMEM69 in these pathways highlighting its involvement in critical cellular processes.
TMEM69 exhibits connections to neurodegenerative diseases and cardiovascular disorders. Altered expression of this protein in brain tissues links it to Alzheimer's disease where it may affect neural cell survival through the PI3K/AKT pathway. In the context of heart disease TMEM69 interacts with proteins such as PI3K suggesting a role in cardiac cell response to stress. These links mark TMEM69 as a protein of interest for disease studies related to neurological and cardiac health.