Abcam, ab263394, Human TNK1 knockout HeLa cell lysate

Size: 1Kit
TNK1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TNK1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tnk1 also known as Tyrosine kinase non-receptor 1 or ACK1 is a non-receptor tyrosine kinase with a molecular mass of approximately 72 kDa. This protein plays a role in signal transduction processes by phosphorylating various substrates. Tnk1 finds expression in different tissues with higher levels observed in the brain heart and testes. Its expression is vital for cellular communication and signaling cascades affecting a variety of cellular functions.
Biological function summary
Tnk1 is involved in regulatory mechanisms that control cell growth and apoptosis. Tnk1 is not part of a well-defined complex but it interacts with various cellular proteins affected by phosphorylation. It has a role in regulating signal pathways that influence gene expression contributing to cellular responses to external stimuli. The ability of Tnk1 to modulate these processes highlights its importance in maintaining cellular homeostasis.
Pathways
Tnk1 integrates into signaling networks such as the epidermal growth factor receptor (EGFR) pathway and Ras signaling pathway. In these pathways Tnk1 interacts with proteins like Epidermal Growth Factor Receptor (EGFR) and Ras assisting in the transduction of extracellular signals that govern cell proliferation and survival. The activity of Tnk1 within these pathways ensures that cells respond appropriately to environmental cues coordinating complex cellular responses.
Tnk1 has been implicated in various cancers including lung and breast cancer. Abnormal expression or mutations in Tnk1 may lead to deregulated cell proliferation contributing to oncogenesis. Associations with other proteins like EGFR further define its role in cancer progression. Understanding the involvement of Tnk1 in these illnesses can provide insights into potential therapeutic approaches highlighting its relevance in disease mechanisms and treatment strategies.