Abcam, ab263398, Human TRAPPC2L knockout HEK-293T cell lysate

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TRAPPC2L KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TRAPPC2L, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRAPPC2L also known as trafficking protein particle complex 2L is a protein approximately 52 kDa in mass. It plays a role in vesicle-mediated transport processes. TRAPPC2L is widely expressed in various tissues but shows higher expression in the adrenal gland and testis. Its mechanical function involves the regulation of intracellular protein trafficking which is important for transporting proteins to their proper destinations within cells.
Biological function summary
This protein is a component of the TRAPP complex a multi-subunit protein assembly involved in the tethering of transport vesicles before fusion with their target membranes. TRAPPC2L contributes to the function and structural integrity of this complex which is essential for maintaining cellular organization and exchanging materials between different cellular compartments. This involvement implies that TRAPPC2L plays a significant part in cellular logistics and maintenance of homeostasis.
Pathways
TRAPPC2L engages in the secretory pathway interfacing with the SNARE proteins necessary for vesicle fusion. This protein also links to the Ypt/Rab GTPases pathway which regulates vesicular transport and budding processes. Through these interactions TRAPPC2L associates with proteins like TRAPPC2 and TRAPPC3 enhancing vesicle tethering and trafficking dynamics.
TRAPPC2L shows associations with medical conditions such as congenital disorders of glycosylation and nephrotic syndrome. Deficient or dysfunctional TRAPPC2L can disrupt vesicle transport pathways leading to cellular dysfunction and disease. The pathways connected to TRAPPC2L can also involve other proteins like TRAPPC2 and TRAPPC9 which contribute to the pathology through their interconnected roles in similar transport processes.