Abcam, ab263412, Human USP54 knockout HeLa cell lysate

Size: 1Kit
USP54 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 107 bp insertion in exon5.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 107 bp insertion in exon5.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-USP54, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
USP54 also known as Ubiquitin-Specific Protease 54 is a deubiquitinating enzyme. It is part of the large family of proteases that removes ubiquitin from proteins marked for degradation therefore regulating their stability. The molecular weight of USP54 is approximately 192 kDa. Expression of USP54 occurs in a range of tissues with higher levels seen in some brain areas and liver. It has widespread but varied activity depending on cellular needs and conditions.
Biological function summary
USP54 plays a role in modulating protein turnover and recycling through its deubiquitinating activity. This regulation helps cells maintain balance in protein levels and activity. Unlike some other deubiquitinating enzymes USP54 is not known to be a component of a larger protein complex. However it shares functional similarities with other enzymes in the ubiquitin-proteasome system which orchestrates the targeted degradation of proteins to control cellular processes.
Pathways
USP54 engages in the ubiquitin-proteasome pathway a critical pathway for protein degradation. This pathway ensures timely removal of misfolded or damaged proteins. USP54 may interact with proteins involved in this pathway to fine-tune the degradation process. Within the context of cellular regulatory networks USP54 may also have connections with signaling pathways where regulation of protein levels impacts signaling cascades. For instance proteins like UBE2D3 and UBC are ubiquitin-conjugating enzymes that might be related through shared pathway dynamics.
USP54 exhibits links to certain cancers and neurological disorders. Its role in protein regulation suggests impacts on cancer through protein stability potentially affecting cell growth and survival. Aberrant expression of USP54 may lead to altered degradation of oncogenic proteins. In neurological disorders USP54's higher expression in brain areas might suggest involvement in processes important for neural maintenance or development. The regulatory actions of USP54 might relate to proteins like p53 a known tumor suppressor protein by influencing its degradation and stability in cancer pathways.