Abcam, ab263420, Human WDR83OS (C19orf56) knockout HEK-293T cell lysate

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WDR83OS KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon 1 and 8 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon 1 and 8 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-WDR83OS, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C19orf56 also known as MONA or INIP functions as an important player in DNA repair processes specifically homologous recombination. It has a molecular weight of approximately 26 kDa. This protein shows expression across various tissues but appears more prominently in the testis and thymus. As part of DNA repair machinery C19orf56 participates in maintaining genome stability by facilitating accurate DNA exchange during cell division.
Biological function summary
The maintenance of genome integrity involves the activity of C19orf56. C19orf56 operates within the DNA damage response and associates with other components to form repair complexes. It is necessary for effective repair of double-strand breaks which protects cells from accumulating mutations. The protein acts in coordination with diverse repair enzymes and structural proteins to detect and correct DNA damage ensuring genomic stasis.
Pathways
C19orf56 serves an essential role in the homologous recombination repair pathway. It interacts closely with proteins like RAD51 an important partner within this pathway. The protein also contributes to the DNA damage signaling pathway where it may interact with p53 an important regulator of the cell cycle and apoptosis. This shows the protein's function in important cellular mechanisms governing cell division and stability.
C19orf56 shows connections to conditions like cancer and Fanconi anemia. Alterations in its function can lead to genomic instability increasing cancer susceptibility. Its interaction with BRCA1 indicates a potential impact on breast cancer pathology where ineffective repair pathways may facilitate oncogenic transformations. Also defects in C19orf56 have associations with impaired bone marrow function seen in Fanconi anemia linking the protein to hematopoietic disorders.