Abcam, ab263425, Human YDJC knockout HEK-293T cell lysate

Size: 1Kit
YDJC KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-YDJC, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
YDJC also known as Tyrosyl-DNA phosphodiesterase 2 (TDP2) is an enzyme involved in repairing DNA damage by removing covalent protein-DNA adducts. This protein has a mass of approximately 66 kDa. YDJC is expressed in various tissues throughout the body including liver kidney and brain. It plays an essential role in maintaining cellular genomic integrity by enabling the repair of trapped topoisomerase-DNA complexes which can otherwise lead to replication fork stalling or double-strand break formation.
Biological function summary
YDJC contributes to cellular DNA repair processes a critical function for cell survival and prevention of mutations. It is not a part of a larger complex functioning independently in its role of cleaving 5’-phosphotyrosyl bonds formed between topoisomerase II and DNA. By facilitating the removal of these protein-DNA lesions YDJC allows the cell to proceed with accurate replication and transcription processes ensuring stability in the genome.
Pathways
YDJC operates within the DNA damage response and repair pathways. Specifically it plays a role in the non-homologous end joining (NHEJ) pathway important for repairing double-strand breaks. YDJC interacts with other proteins such as DNA-PKcs and Ku during this repair process. In addition it is related to the cell cycle regulation pathway where its function is to prevent the accumulation of DNA damage therefore aiding in maintaining cell cycle integrity.
YDJC has connections to cancer and neurodegenerative diseases. In cancer the efficient repair of DNA damage by YDJC can influence tumor resistance to certain chemotherapeutic agents. Aberrant expression of YDJC can lead to increased DNA damage resistance in tumor cells potentially leading to chemotherapy resistance. Furthermore in neurodegenerative diseases the failure to repair DNA damage due to impaired YDJC function may contribute to neuronal cell death. YDJC's activity links it to the XRCC1 protein which is involved in maintaining neuronal health through DNA repair mechanisms.