Abcam, ab263426, Human YIF1A knockout HEK-293T cell lysate

Size: 1Kit
YIF1A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-YIF1A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
YIF1A also known as Yip1 Family Member 1 or Yin Yang 1-Interacting Factor 1 functions mechanically as a membrane protein involved in trafficking between the endoplasmic reticulum (ER) and the Golgi apparatus. It has a molecular mass of approximately 35 kDa. This protein localizes predominantly to intracellular membranous structures most notably within the ER and the Golgi. Its expression occurs in various tissues including brain liver and kidney where it plays specialized roles in protein transport and cellular homeostasis.
Biological function summary
This protein interacts with various components of intracellular transport machinery. YIF1A is part of a larger YIP1 complex that supports vesicle formation and trafficking essential for maintaining proper cellular function. Through its interaction with this complex YIF1A contributes to the regulation of protein sorting and distribution which is necessary for functional membrane dynamics and signal transduction.
Pathways
YIF1A participates in intracellular transport and vesicle-mediated processes. It associates with the vesicular trafficking pathway where it regulates the movement and sorting of proteins between the ER and Golgi. YIF1A works in conjunction with proteins like YIPF5 in these pathways which coordinate the accurate delivery and modification of proteins necessary for normal cellular operations.
YIF1A's dysregulation links to neurodegenerative diseases such as Parkinson's disease. Misfunction in YIF1A can lead to improper protein trafficking contributing to cellular stress and neuron degeneration. Additionally YIF1A connects to the sigma-1 receptor a protein implicated in amyotrophic lateral sclerosis (ALS) indicating its relevance in neurological health. Understanding these associations can offer insights into potential therapeutic targets for these ailments.