Abcam, ab263871, Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal BAF170 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23066-21,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, ICC/IF, Flow Cyt (Intra), IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab263871 is the carrier-free version of
ab243634
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
BAF170 also known as SMARCC2 is a protein that plays an essential role in chromatin remodeling. The molecular mass of BAF170 is approximately 131 kDa. It is expressed in various tissues including the brain and heart where it helps in altering chromatin structure to allow for transcriptional regulation. The protein contributes to the regulation of gene expression by modulating the accessibility of chromatin-bound transcription factors.
Biological function summary
BAF170 forms an integral part of the SWI/SNF chromatin remodeling complex which influences how genes are expressed in the cell. This complex alters the position of nucleosomes enabling the access of transcriptional machinery to DNA. Through this action BAF170 regulates the expression of genes necessary for processes such as cell differentiation and proliferation. The SWI/SNF complex interacts with multiple other proteins creating a dynamic regulatory environment for gene expression.
Pathways
BAF170 participates in significant cellular pathways like the Wnt signaling pathway and the Hedgehog pathway. These pathways influence many vital cellular processes including development and cell cycle regulation. In the Wnt pathway BAF170 works in concert with other proteins such as beta-catenin to regulate target gene expression. Similarly in the Hedgehog pathway BAF170 interacts with proteins involved in the signaling cascade affecting cellular responses and developmental processes.
Aberrations in BAF170 activity associate with certain cancers and neurodevelopmental disorders. Alterations in the SWI/SNF complex's function where BAF170 is a central component can result in malignant transformations leading to tumors. Moreover disruptions in BAF170's regulation are linked to issues impacting brain development possibly influencing conditions such as autism spectrum disorders. Researchers continue to investigate the protein's role in other pathological states providing insight into its contribution to disease mechanisms.