Abcam, ab264688, Human RIPK2 (RIP2) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
RIPK2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 2 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 2 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RIPK2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RIP2 also known as receptor-interacting serine/threonine-protein kinase 2 or RICK is a protein with roles in signal transduction. It functions mechanically as a kinase which means it adds a phosphate group to other proteins impacting their activity and stability. RIP2 weighs about 61 kDa and is mostly expressed in immune system-related tissues such as spleen thymus peripheral blood leukocytes and bone marrow. As a kinase RIP2 is an action part in the cellular response to external stimuli.
Biological function summary
RIP2 interacts with key components of the immune signaling network. It forms part of a complex with other proteins like nucleotide-binding oligomerization domain (NOD) proteins influencing immune responses. By binding to NOD1 and NOD2 RIP2 modulates inflammatory signaling pathways leading to the activation of NF-kB and the production of inflammatory cytokines. This protein helps regulate the body's defense against bacterial infections and influences inflammation processes.
Pathways
RIP2 plays an integral role in the NOD-like receptor signaling pathway and is also involved in toll-like receptor signaling. In its pathways RIP2 interacts closely with proteins like IKK complexes and the Tak1 complex orchestrating signaling cascades leading to the activation of NF-kB and MAPK pathways. These pathways are essential for instigating the immune response and managing inflammatory signals highlighting how RIP2 acts as a pivotal mediator in these biological routes.
Mutations or dysregulation of RIP2 are linked to inflammatory diseases and immune disorders such as Crohn's disease and Blau syndrome. RIP2's involvement in these conditions often involves its interaction with NOD2 influencing the improper immune response seen in these diseases. Changing RIP2 activity presents a target for developing kinase inhibitors that might help to manage inflammation and immune system disorders by modulating its signaling activity.