Abcam, ab264761, Human RRAGB knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
RRAGB KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 85 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 85 bp insertion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RRAGB, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RRAGB also known as Ras-related GTPase B is a member of the Rag family of GTPases. This protein has a molecular mass of approximately 42 kDa. RRAGB is expressed in various tissues with significant expression in the brain and skeletal muscles. The protein functions as a molecular switch toggling between active GTP-bound and inactive GDP-bound states. This cycling allows RRAGB to regulate mTORC1 signaling which is essential for cellular growth and metabolism.
Biological function summary
RRAGB partners with other Rag GTPases to form heterodimeric complexes. These complexes facilitate the proper localization and activation of mTORC1 on the lysosomal membrane. Moreover RRAGB plays a role in amino acid sensing which impacts cellular growth. By responding to intracellular nutrient levels RRAGB helps modulate anabolic processes and energy balance.
Pathways
RRAGB is integral to the mTOR signaling and nutrient-sensing pathways. As a part of these pathways RRAGB collaborates with proteins such as Raptor and Rheb. These interactions ensure efficient transmission of signals that regulate cell growth and metabolism. The accurate function of this network supports cellular adaptation to varying environmental nutrient conditions.
RRAGB has been associated with certain cancers and neurodegenerative diseases. Alterations in the mTOR pathway involving RRAGB can contribute to undesired cellular proliferation observed in cancers. Moreover disruptions in RRAGB activity can influence mTORC1 signaling in neurodegenerative conditions. It's important to further understand how RRAGB and related proteins like mTOR are involved in the pathology of these diseases to develop targeted therapies.