Abcam, ab264840, Human HIPK3 (FIST) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
HIPK3 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-HIPK3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The FIST protein also known as Found in Squamous Cell Carcinoma Transporter possesses a mass of approximately 60 kDa. It functions mechanically as a transporter protein facilitating the movement of molecules across cellular membranes. Expression of FIST occurs in various tissues with particular abundance in squamous cell carcinoma cells where it plays a role in tumor cell survival and proliferation.
Biological function summary
FIST contributes to cellular processes by regulating the transport of specific substrates such as ions and small molecules critical for cellular homeostasis and energy balance. This protein is often part of a larger transporter complex allowing it to interact with other proteins to modulate its transport activities efficiently. Its role becomes more significant in squamous cell carcinoma where altered transport activities could contribute to the development and progression of the cancer.
Pathways
FIST has significant involvement in the cellular energy metabolism and signaling pathways. One key pathway is the ion transport pathway where FIST interacts with several channel proteins to maintain ionic concentration and cellular excitability. Moreover it associates with ATP-binding cassette (ABC) transporters participating in the regulation of multidrug resistance which provides cancer cells with a mechanism to survive chemotherapeutic treatments.
FIST has associations with squamous cell carcinoma and an increased risk of resistance to cancer therapies. Alterations in FIST expression or function can disrupt cellular ion homeostasis contributing to tumor development and progression. The protein links to the disease through its interaction with channel proteins affecting cell proliferation. Additionally its collaboration with ABC transporters in multidrug resistance pathways impacts the effectiveness of chemotherapy making the study of FIST critical in understanding and combating squamous cell carcinoma.