Abcam, ab264851, Human SCFD2 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SCFD2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SCFD2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SCFD2 also known as Sec1 family domain-containing protein 2 is a protein with a mass of approximately 42 kDa. It functions as an essential component in vesicle-mediated transport processes. SCFD2 is highly expressed in the brain testis and liver which indicates its potential relevance in multiple organ systems. Mechanically SCFD2 plays an active role in vesicle docking and fusion events mainly by interacting with SNARE complexes facilitating the release of vesicle contents at the target membrane.
Biological function summary
The function of SCFD2 is closely linked with the regulation of neurotransmitter release and protein trafficking within cells. Though it does not act alone SCFD2 associates with the exocyst complex involving several additional proteins critical for the proper localization and functioning of vesicles. This coordination ensures efficient cellular communication and contributes to normal cellular processes such as synaptic transmission and intracellular signaling.
Pathways
The role of SCFD2 extends into the vesicular transport and exocytosis pathways. It assists in the functioning of the SNARE machinery important for these pathways by interacting with proteins like SNAP-25 and syntaxin. These interactions highlight SCFD2's contribution to the dynamics of vesicle movement and release which are fundamental aspects of cellular homeostasis and responsiveness.
Disruptions or mutations in SCFD2 have connections with neurological conditions and neurodegenerative diseases including Alzheimer's disease. Its association with Alzheimer's is partly mediated through its relationship with other key proteins involved in the disease's pathways such as amyloid precursor protein (APP) and tau. Research into SCFD2 continues to clarify its precise roles in various diseases suggesting its potential as a therapeutic target.