Abcam, ab265106, Human PPP1R3C knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PPP1R3C KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PPP1R3C, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PPP1R3C also known as protein phosphatase 1 regulatory subunit 3C is a regulatory subunit of protein phosphatase 1 (PP1). This protein has a mass of approximately 34 kDa. PPP1R3C is mainly expressed in skeletal muscle and liver tissues. It acts by modulating glycogen metabolism where it impacts glycogen synthesis and breakdown. This regulation is achieved through altering the activity of PP1 which dephosphorylates diverse biological substrates to control various cellular functions.
Biological function summary
The PPP1R3C protein binds to glycogen and PP1 forming a complex with these substrates. This interaction plays a role in the regulation of glucose homeostasis by controlling glycogen synthesis and breakdown. Such processes are important for energy storage and release in muscle and liver tissues. By integrating within this complex PPP1R3C influences the activity and localization of PP1 facilitating efficient glycogen regulation.
Pathways
PPP1R3C participates in critical pathways like glycogen metabolism and insulin signaling. The protein is closely associated with key metabolic proteins such as glycogen synthase. Within these pathways PPP1R3C modifies the enzymatic activity of its pathway partners to balance glucose levels in response to insulin. This involvement supports metabolic flexibility and energy management in response to dietary changes.
PPP1R3C connects to insulin resistance and type 2 diabetes where its altered function may disrupt normal glycogen metabolism. The protein's dysfunction impacts glucose homeostasis and insulin signaling pathway contributing to the pathophysiology of these metabolic diseases. Additionally PPP1R3C interacts with proteins like glycogen synthase which play significant roles in the metabolic disturbances seen in such disorders. Understanding these interactions helps in devising strategies for targeted therapeutic interventions.