Abcam, ab265141, Human PGAM5 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PGAM5 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1,
Antibiotic resistance:Puromycin 1µg/mL,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PGAM5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PGAM5 also known as Phosphoglycerate Mutase Family Member 5 is a mitochondrial protein with a known mass of around 32 kDa. It acts as a serine/threonine phosphatase without intrinsic catalytic phosphoglycerate mutase activity. PGAM5 localizes to the inner membrane of mitochondria and exhibits expression in tissues where metabolic regulation is significant. It plays a role in removing phosphate groups from target substrates influencing multiple cellular mechanisms.
Biological function summary
PGAM5 impacts pathways related to cellular stress responses and energy metabolism. It participates in programmed cell death and modulates mitochondrial dynamics. It can serve as part of a complex with other proteins adjusting the balance between mitophagy and apoptosis. Through interaction with key signaling molecules PGAM5 bridges various biochemical processes relevant to cell survival and energy utilization.
Pathways
Its function integrates into mitophagy and necroptosis. PGAM5 collaborates with proteins like PINK1 and PARKIN contributing to the clearance of damaged mitochondria via the mitophagy pathway. In necroptosis PGAM5 interacts with RIPK1 and RIPK3 regulating necrotic cell death. These interactions position PGAM5 at the crossroads of pathways critical to maintaining cellular homeostasis and response to stress.
PGAM5 relates to neurodegenerative diseases and cancer. Mutations or dysregulation of PGAM5 connect to Parkinson's disease where impaired mitochondrial function and mitophagy play a role. In cancer altered PGAM5 activity may disrupt normal apoptotic processes leading to unchecked cell growth. In these contexts PGAM5's interaction with proteins like PINK1 PARKIN RIPK1 and RIPK3 underpins its relevance to disease mechanisms.