Abcam, ab265155, Human PTBP1 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PTBP1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 4,
Antibiotic resistance:Puromycin 1µg/mL,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PTBP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PTBP1 also known as polypyrimidine tract-binding protein 1 or hnRNP I is a protein involved in RNA binding. It weighs approximately 57 kDa and is known for its expression in diverse cell types especially in the nucleus of eukaryotic cells. PTBP1 contains four RNA recognition motifs which allow it to interact with polypyrimidine-rich sequences in pre-mRNA. This interaction plays an important role in RNA splicing and the regulation of alternative splicing events.
Biological function summary
PTBP1 influences the stability transport and translation of mRNA molecules. It is part of the heterogeneous nuclear ribonucleoprotein complex which plays a fundamental role in the processing and modification of pre-mRNA into mature mRNA. This activity impacts the expression of a wide range of genes affecting cellular function and growth. PTBP1 regulates mRNA metabolism contributing significantly to post-transcriptional gene regulation.
Pathways
The involvement of PTBP1 spans the management of alternative splicing and the modulation of gene expression. It participates importantly in the alternative splicing pathway alongside proteins like PTBP2 which perform overlapping functions in neurons. PTBP1 affects exon inclusion or exclusion influencing the genetic diversity presented in protein forms within cells. It also plays a role in the exon junction complex pathway impacting post-translational modifications and protein interactions.
Variations in PTBP1 function are linked to conditions such as cancer and neurological disorders. Altered expression levels or mutations can contribute to carcinogenesis through the misregulation of splice variants specific to oncogenes and tumor suppressor genes. In neurological disorders PTBP1 interacts with proteins like FUS affecting the regulation of neuronal-specific alternative splicing. Misbalance in these interactions can have significant pathological implications contributing to diseases such as amyotrophic lateral sclerosis (ALS) and glioblastomas.