Abcam, ab265226, Human FER knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
FER KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 4,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-FER, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The FER protein also known as mouse FER or FER-1 functions as a non-receptor tyrosine kinase within the Fes/Fps subfamily. It has a molecular mass of approximately 94 kDa. FER is expressed across various tissues notably in epithelial cells and is also present in hematopoietic and neural cells. Its kinase activity regulates cell signaling affecting processes like cell proliferation differentiation and survival.
Biological function summary
FER acts as a regulator in signaling pathways essential for cellular communication and structure maintenance. FER interacts within cellular complexes modulating the cytoskeleton and intercellular junctions. It influences actin remodeling impacting cell adhesion and motility. FER's involvement in such activities makes it important for maintaining epithelial integrity and cellular response to external stimuli.
Pathways
FER plays a role in the PI3K/Akt pathway and the MAPK/ERK pathway. These pathways are important for cell growth survival and proliferation. FER interacts with other proteins like STAT3 and JAK2 integrating signals that drive these pathways. By modulating key signaling events FER influences cellular functions and responses to environmental changes.
FER associates with cancer and cardiovascular diseases. Aberrant FER expression and activity have been found in various tumors linking it to cancer progression and metastasis. In cardiovascular disease FER's role in regulating endothelial function connects it to vascular abnormalities. Its interaction with proteins like VEGFR further implicates FER in the pathogenesis of these conditions serving as a potential therapeutic target.