Abcam, ab265229, Human RTN4RL2 (NgR2) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
RTN4RL2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp deletion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RTN4RL2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NgR2 also known as Nogo receptor 2 or Reticulon 4 receptor 2 is a glycosylphosphatidylinositol (GPI)-anchored protein with a molecular mass of approximately 55 kDa. It is mainly expressed in the central nervous system where it functions as an axon guidance receptor. NgR2 binds to myelin-associated proteins including myelin-associated glycoprotein (MAG) oligodendrocyte myelin glycoprotein (OMgp) and Nogo-A inhibiting axon regeneration in the adult nervous system. This protein is important for maintaining neuronal architecture and regulating neuronal growth.
Biological function summary
The action of NgR2 impacts the neural regeneration processes by forming a receptor complex with p75 neurotrophin receptor (p75NTR) and LINGO-1. This complex activation inhibits axonal outgrowth and neural plasticity which affects the nervous system's ability to recover after injury. NgR2 mediates signaling that prevents neurite outgrowth contributing to the stabilization of neuronal connections in postnatal brain development. NgR2's role in these processes makes it a significant target for studies investigating neuronal repair mechanisms.
Pathways
Axon guidance and myelin inhibition play significant roles in the function of NgR2. It participates in the RhoA-ROCK signaling pathway which is activated upon myelin ligand binding to the receptor complex. This pathway is responsible for cytoskeletal changes that inhibit neurite extension. Additionally NgR2 interacts with proteins such as Nogo-A and MAG further influencing axonal growth inhibition and regeneration in the nervous system.
Defects in NgR2 function or expression can relate to conditions such as spinal cord injury and multiple sclerosis. These disorders exhibit impaired neural repair and regeneration where NgR2 and its associated proteins like Nogo-A and MAG play a role in limiting recovery and repair mechanisms. Understanding the interactions and regulation of NgR2 in these contexts can provide insights into therapeutic approaches aimed at enhancing neural regeneration and functional recovery.