Abcam, ab265238, Human MMRN1 (ECM) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
MMRN1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1 and Insertion of the selection cassette in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MMRN1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The extracellular matrix (ECM) constitutes a complex network of macromolecules such as proteins and glycoproteins including the key structural proteins like collagen elastin fibronectin and laminin. It provides structural support to cells and tissues and plays a role in signal transmission across the cellular environment. The ECM does not have a singular mass due to its diverse composition but is expressed throughout different tissues in the body. Its complexity and vast distribution are essential for maintaining tissue architecture and function.
Biological function summary
The extracellular matrix coordinates cellular functions by mediating mechanical and biochemical signals influencing cellular processes such as adhesion differentiation and migration. The ECM is an essential component of the matrix receptor complex interacting dynamically with cell-surface receptors like integrins. These interactions are important to tissue remodeling and repair allowing tissues to maintain homeostasis and respond to external stresses.
Pathways
The extracellular matrix participates in significant biological pathways like the integrin signaling pathway and the Wnt signaling pathway. These pathways link ECM to various cellular responses affecting processes such as cell migration proliferation and survival. Integrin proteins for example bind to ECM proteins transmitting signals that can regulate gene expression and determine cell fate. Disruption in these pathways has implications for developmental processes and disease states.
The extracellular matrix plays a role in conditions such as fibrosis and cancer. Alterations in ECM components or remodelling can lead to fibrotic diseases where excessive ECM deposition affects tissue function. In cancer ECM changes can influence tumor progression and metastasis. Matrix metalloproteinases (MMPs) a group of enzymes breaking down ECM proteins connect ECM to these pathological states altering tissue integrity and facilitating disease progression. Understanding ECM dynamics is therefore essential for developing therapeutic strategies against these disorders.