Abcam, ab265285, Human PUM1 (Pumilio 1) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PUM1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 121 bp insertion in exon 2 and 1 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 121 bp insertion in exon 2 and 1 bp deletion in exon 2,
Antibiotic resistance:Puromycin 1µg/mL,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PUM1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pumilio 1 also known as PUM1 is a human protein coding for RNA-binding protein involved in the translational regulation of mRNA. It has a molecular weight of approximately 120 kDa. PUM1 expresses in various tissues with higher concentrations in brain placenta and testis. This protein contains a distinct Pumilio Homology Domain which allows it to specifically recognize and bind to a conserved sequence motif known as Pumilio Response Element (PRE) in target mRNAs.
Biological function summary
PUM1 is an important regulator of gene expression. It functions by binding to the 3' untranslated regions of target mRNAs leading to repression of translation and in some cases RNA decay. PUM1 operates within larger ribonucleoprotein complexes collaborating with other regulatory proteins to fine-tune the stability and translation of specific transcripts. This regulation is vital for processes like neuronal development stem cell maintenance and germ cell development.
Pathways
The influence of PUM1 extends to key biological networks such as the cell cycle and long-term potentiation pathways. It interacts with proteins like DDX6 which synergize to mediate post-transcriptional regulation as part of the mRNA decay pathway. Through its role in these pathways PUM1 helps ensure precise control of gene expression necessary for cellular homeostasis and adaptive responses to environmental changes.
The dysfunction of PUM1 is linked to neurological and reproductive conditions. Mutations or alterations in PUM1 expression have associations with spinocerebellar ataxia and gonadal dysgenesis. In these contexts PUM1 aberrations may also involve interactions with proteins such as FMRP known for its roles in neurological development and reproductive processes pointing to a complex network of genetic regulation and disease manifestation.