Abcam, ab265335, Human PERP knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PERP KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 70 bp insertion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 70 bp insertion in exon 1 and Insertion of the selection cassette in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PERP, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PERP also known as P53 apoptosis effector related to PMP-22 is a protein with an approximate mass of 23 kDa. It mainly operates as an effector in the p53-dependent apoptosis pathway. PERP is known for facilitating cell-cell adhesion as a part of the desmosome complex. Researchers have found its expression primarily in epithelial tissues where it contributes to maintaining cell structure integrity. PERP's role is significant in tissues experiencing stress where it upregulates in response to p53 activation.
Biological function summary
PERP plays an active role in cellular processes like adhesion and apoptosis. As a part of the desmosome it helps in connecting epithelial cells. This function is important for preserving tissue architecture and responding to mechanical stress. PERP's involvement in apoptosis arises from its interactions in the p53 pathway supporting the process when cells require programmed death. These roles are essential for maintaining balanced cell proliferation and death key for tissue homeostasis.
Pathways
PERP is closely tied to the p53 pathway and the desmosomal adhesion process. The p53 pathway involves one of the primary tumor suppressor proteins and regulates the cell cycle while the desmosomal adhesion pathway maintains the structural cohesion between cells. PERP acts in concert with various proteins such as JUP (Plakoglobin) strengthening cellular adhesions and responding to apoptotic signals. This connectivity allows PERP to perform dual roles in maintaining cell stability and facilitating apoptosis when necessary.
PERP has associations with squamous cell carcinoma and epidermolysis bullosa. Disruption of PERP functions can lead to improper apoptosis contributing to cancer progression. In the case of epidermolysis bullosa alterations in desmosomal structure connected to PERP can weaken skin integrity leading to blistering. In these conditions proteins like JUP work alongside PERP highlighting the importance of balance between cell adhesion and controlled cell death in disease prevention.