Abcam, ab265371, Human RAB31 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
RAB31 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RAB31, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RAB31 also known as RAB22B belongs to the RAB family which plays an important role in vesicular trafficking. It is a small GTPase with a mass of approximately 23 kDa. RAB31 is mainly expressed in epithelial tissues and certain cancer cell lines. It regulates vesicle formation movement and fusion by cycling between an active GTP-bound state and an inactive GDP-bound state. This cycling is important for its function in the intracellular transport system.
Biological function summary
RAB31 influences early endosome dynamics and directly impacts the sorting and recycling of receptors. It associates with effector proteins to form protein complexes that facilitate endocytosis and exocytosis processes. It also interacts with other Rab proteins to ensure proper membrane specificity and temporal regulation within cellular compartments. This behavior highlights its role in maintaining cellular homeostasis and responsiveness to environmental changes.
Pathways
Studies have shown that RAB31 contributes significantly to the endocytic pathway and the insulin signaling pathway. It has functional interactions with proteins like RAB5 and RAB11 both of which are key regulators of endosomal trafficking. RAB31 ensures a proper transport cycle that influences receptor recycling and signaling impacting how cells respond to growth factors and hormones.
Research implicates RAB31 in the progression of certain cancers such as breast cancer due to its established role in vesicle trafficking and receptor recycling which can affect cell growth and division. Its altered expression levels may intersect with proteins such as EGFR leading to abnormal signaling cascades. Furthermore RAB31 has associations with metabolic disorders as disruptions in insulin signaling pathways may contribute to diabetes showcasing its diverse influence on human health.