Abcam, ab265470, Human POLR1D knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
POLR1D KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-POLR1D, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
POLR1D also known as RNA polymerase I and III subunit D is a component of the RNA polymerase I and III complexes. It weighs approximately 16.5 kDa. This protein is important for the transcription of ribosomal RNA (rRNA) and small RNA molecules. It is primarily expressed in cells that have high transcriptional activity such as in tissues like the pancreas thymus and spleen.
Biological function summary
The activity of POLR1D contributes to the formation of ribosomal RNA an essential component of the ribosome which is responsible for protein synthesis. POLR1D forms part of both RNA Polymerase I and RNA Polymerase III complexes which are responsible for transcribing rRNA and other small RNAs respectively. It interacts closely with other subunits within these complexes to facilitate transcription processes.
Pathways
POLR1D plays a role in the rRNA transcription pathway and the RNA polymerase I promoter opening pathway. Its function is essential within these pathways to initiate and sustain the transcription required for ribosome production. POLR1D is related to other subunits of RNA polymerases like POLR1A and POLR1B through these pathways forming an interconnected network essential for nucleolar function and RNA maturation.
Malfunction or mutations within POLR1D can lead to Treacher Collins syndrome which is a disorder affecting craniofacial development. This connection highlights the importance of properly functioning RNA polymerases for developmental processes. Additionally disturbances in POLR1D have been studied in relation to leukemias where disrupted transcription processes can contribute to uncontrolled cell proliferation and cancer. POLR1D's role in these diseases highlights its importance in maintaining normal cellular operation and development.