Abcam, ab265539, Human PKP3 (Plakophilin 3) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PKP3 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PKP3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Plakophilin 3 often referred to as PKP3 is a member of the Armadillo family of proteins. PKP3 is a desmosomal plaque protein with a known molecular mass of approximately 84 kDa. It localizes primarily to desmosomes and the nucleus where it contributes to cell-cell adhesion. PKP3 is commonly expressed in epithelial tissues where it helps maintain the structural integrity of epithelial sheets by binding to desmosomal cadherins.
Biological function summary
Plakophilin 3 plays a vital role in stabilizing junctional complexes by anchoring desmosomal components to the cytoskeleton. It usually forms a part of the desmosome a cellular structure composed of desmogleins desmocollins and plakoglobin. Through these interactions PKP3 contributes to the robust mechanical strength of tissue architecture and participates in signaling pathways that regulate cell proliferation and differentiation.
Pathways
Plakophilin 3 integrates into desmosome-related signaling pathways notably the intercellular adhesion pathways and the Wnt signaling pathway. PKP3 associates with proteins such as plakoglobin and β-catenin which are also involved in Wnt signaling influencing cell-cell contact and transcriptional regulation. These pathways are important for maintaining the balance between cell adhesion and cellular signaling ensuring appropriate responses to developmental and environmental cues.
Plakophilin 3 shows a connection to certain forms of cancer including prostate cancer and squamous cell carcinoma. PKP3 expression levels have an association with tumor progression and metastasis. In prostate cancer for instance altered expression of desmosomal components such as PKP3 can influence cancer cell adhesion and mobility. PKP3 interacts with proteins like E-cadherin in these pathological states which can affect adhesion properties and promote malignancy.