Abcam, ab265558, Human PSMD5 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PSMD5 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PSMD5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PSMD5 also known as S13 or 26S proteasome non-ATPase regulatory subunit 5 plays an essential role in the ubiquitin-proteasome system. It has a molecular mass of approximately 39 kDa. PSMD5 is a component of the 26S proteasome complex a structure important for protein degradation in cells. This protein locates in various tissues especially in high-protein turnover areas such as the liver skeletal muscle and heart.
Biological function summary
The protein influences the breakdown of ubiquitinated proteins. PSMD5 forms part of the 19S regulatory subunit of the 26S proteasome complex. The 19S regulatory complex is significant for recognizing and unfolding ubiquitinated substrates guiding their entry into the proteolytic 20S core. By participating in this process PSMD5 aids in maintaining protein quality control and regulating the cell cycle.
Pathways
PSMD5 affects the ubiquitin-proteasome degradation pathway an important regulator of protein turnover. This pathway is essential for numerous cellular processes including cell cycle progression and signal transduction. PSMD5 interacts with proteins such as ubiquitin and other subunits of the proteasome complex to facilitate the degradation of proteins tagged for destruction. It also plays a part in pathways linked to NF-kB signaling where it may interface with proteins like IκB a known inhibitor of the NF-kB pathway.
PSMD5 connects to conditions such as cancer and neurodegenerative diseases. The protein's role in the regulation of protein degradation implicates it in diseases where protein homeostasis goes awry. For example alterations in proteasome activity can lead to the accumulation of damaged proteins a hallmark of neurodegenerative disorders. In cancer dysregulation of protein degradation may contribute to uncontrolled cell growth and survival. Elevated levels of ubiquitin-like proteins often accompany such pathological conditions highlighting PSMD5's involvement.