Abcam, ab265562, Human ITFG1 (CDA08) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
ITFG1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ITFG1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CDA08 also known as cytidine deaminase weighs approximately 16 kDa and is expressed mainly in the spleen liver and bone marrow. This enzyme catalyzes the deamination of cytidine transforming cytidine into uridine and plays a role in nucleotide metabolism. By conducting this reaction CDA08 regulates cytidine and deoxycytidine levels impacting DNA and RNA synthesis.
Biological function summary
The enzyme CDA08 modulates cell proliferation and apoptosis through its regulation of nucleotide pools. It is not part of any known larger protein complex standing as a singular enzyme in its function. CDA08 activity influences the balance between pyrimidine metabolites impacting cellular functions such as DNA repair and replication.
Pathways
Scientists have linked CDA08 to the pyrimidine metabolism and nucleic acid synthesis pathways. Its activity integrates with key enzymes in these processes maintaining cellular nucleotide homeostasis. Additionally proteins like ribonucleotide reductase interact functionally with CDA08 showing a coordinated regulation of deoxycytidine triphosphate levels essential for DNA synthesis.
Researchers have connected CDA08 with acute myeloid leukemia and certain lymphomas. Alterations in CDA08 expression or activity can affect chemotherapeutic drug metabolism influencing treatment outcomes. Furthermore proteins such as deoxycytidine kinase are related to CDA08 through their roles in these disorders linking drug resistance mechanisms with nucleotide metabolism.