Abcam, ab265627, Human FOCAD (Focadhesin) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
FOCAD KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 12. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 12,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-FOCAD, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Focadhesin also known as FDN-1 is a structural protein involved in cell adhesion processes. It has a molecular mass of approximately 50 kDa. Focadhesin primarily expresses in epithelial tissue where it plays a role in maintaining cell junction integrity. Researchers prefer focusing on this target for studies related to tissue development and repair. The solid expression in epithelial cells makes it integral to tissue architecture and stability.
Biological function summary
Focadhesin contributes to cellular adhesion and communication mechanisms. It operates as part of a multiprotein complex associated with adhesion molecules such as cadherins and integrins. This complex facilitates the stabilization of cell junctions supporting structural cohesion between cells. Focadhesin's presence is necessary for processes involving epithelial cell cohesion and barrier function. Its interactions with other adhesion molecules promote the assembly and maintenance of intercellular connections.
Pathways
Focadhesin plays a significant role in cell signaling pathways that control cell adhesion and migration particularly the Wnt and PI3K/AKT pathways. Its interaction with proteins like beta-catenin and phosphoinositide 3-kinase highlights its importance in signaling cascades that affect cellular dynamics and growth. These pathways influence cellular responses and integrity impacting processes like tissue remodeling and stress responses.
Focadhesin's dysfunctional expression has links to epithelial cancers such as breast and colorectal cancer. Altered focadhesin levels may disrupt adhesion and signaling promoting tumor progression and metastasis. It also associates with fibrotic disorders like pulmonary fibrosis where improper adhesion molecule regulation contributes to structural tissue changes. Focadhesin's relationship with proteins such as E-cadherin and TGF-beta reveals its involvement in these pathological conditions making it a target of interest for therapeutic interventions.