Abcam, ab265660, Human SHC4 (SHCD) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SHC4 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 4 and 4 bp deletion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 4 and 4 bp deletion in exon 4,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SHC4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SHC4 also known as SHCD is a member of the SHC (Src Homology 2 domain-containing) family and plays an important role in intracellular signaling. The protein has a molecular mass of approximately 72 kDa. SHC4 mainly localizes in the cytoplasm and can translocate to the membrane where it participates in signaling activities. Its expression occurs widely across different tissues but it shows a particularly strong expression in the nervous system indicating a potential role in neural processes.
Biological function summary
SHC4 serves as an adaptor protein that connects activated receptors to downstream signaling pathways. It can associate with various signaling complexes helping to mediate responses to extracellular stimuli. SHC4 transmits signals from receptors like receptor tyrosine kinases (RTKs) and plays a part in cellular functions such as proliferation and differentiation. These attributes highlight its involvement in complex networks that control cellular responses.
Pathways
SHC4 participates in signaling cascades like the MAPK/ERK and PI3K/AKT pathways. It interacts with proteins such as GRB2 which transmits signals from activated RTKs to the Ras protein ultimately influencing cell fate decisions. These pathways are critical for regulating cell cycle progression cellular growth and metabolism demonstrating SHC4's integral function in these processes.
SHC4 associates with conditions impacting the nervous system including neurodevelopmental disorders. Its altered signaling capability may relate to aberrant neural development affecting processes stabilized by SHC4. Moreover SHC4 has been studied in cancer where dysregulation of associated pathways like MAPK/ERK plays a significant role. Its interaction with proteins such as RAS which can act as oncogenes when mutated marks SHC4 as an important protein in oncology research.