Abcam, ab265665, Human USP9X knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
USP9X KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 9 and 1 bp insertion in exon 9. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 9 and 1 bp insertion in exon 9,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-USP9X, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
USP9x also known as ubiquitin-specific peptidase 9 X-linked is a deubiquitinating enzyme that plays a critical role in protein homeostasis. USP9x belongs to the USP family and has an approximate molecular mass of 290 kDa. It is expressed broadly in human tissues with particularly high levels in the brain and pancreas. Mechanically USP9x functions to remove ubiquitin molecules from ubiquitinated substrates preventing their degradation by the proteasome. This regulatory action influences many cellular processes by stabilizing specific proteins.
Biological function summary
USP9x influences numerous cellular mechanisms by maintaining protein balance. It is a part of the complex cellular machinery involved in signal transduction cell division and apoptosis. While its enzyme activity regulates various cellular proteins USP9x is essential in neural development and differentiation. The proteolytic activity helps to modulate pathways by controlling protein turnover which impacts cellular responses and development. Failure to regulate proteins accurately can lead to compromised cellular functions.
Pathways
USP9x participates prominently in the Wnt signaling and TGF-beta pathways. Its role in deubiquitination allows it to stabilize proteins like beta-catenin and SMAD4 which are vital for transmitting signals within these pathways. Through these interactions USP9x aids in cell proliferation migration and differentiation often connecting with other proteins like APC or Axin in the Wnt pathway. This demonstrates how USP9x's enzymatic activity intricately links to key biological signaling mechanisms.
Mutations and dysregulation of USP9x associate with cancer and neurodevelopmental disorders. In cancer it impairs normal cell cycle control and apoptosis facilitating unchecked cell proliferation. It interacts with proteins such as MCL1 influencing apoptosis decisions and tumor growth. In neurodevelopmental disorders USP9x affects brain development by disrupting neural cell differentiation processes. The protein's malfunction causes imbalances in brain signaling linking it to conditions like intellectual disability.