Abcam, ab265702, Human RABAC1 (PRA1) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
RABAC1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RABAC1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PRA1 protein also known as prenylated Rab acceptor 1 is involved in vesicular transport processes within the cell. This protein has a molecular mass of approximately 21 kDa and plays an important role in the trafficking of proteins bound for various destinations in the secretory pathway. PRA1 is widely expressed in many tissues reflecting its integral function in cellular transport mechanisms.
Biological function summary
PRA1 interacts with Rab GTPases which suggest its role in facilitating the docking or fusion of transport vesicles with target membranes. Although it does not form a complex on its own PRA1 acts as an effector anchoring prenylated Rab proteins. This interaction is essential for maintaining the fidelity and specificity of vesicle transport events necessary for proper cellular functioning.
Pathways
PRA1 participates in the regulation of the Rab GTPase cycle and is important in the endocytic and secretory pathways. It modulates the activity of proteins such as Rab1 and Rab5 that coordinate the movement of vesicles from the endoplasmic reticulum to the Golgi and from the Golgi to the plasma membrane. These pathways are fundamental for cellular logistics ensuring that proteins reach the intracellular or extracellular locations where they are needed.
PRA1 has been linked to neurodegenerative conditions like Alzheimer's disease possibly due to its involvement in vesicular transport and protein trafficking dysregulation. Alterations in PRA1 expression or function can affect proteins such as Rab proteins potentially contributing to the pathological process of such disorders. Another condition associated with PRA1 is Charcot-Marie-Tooth disease suggesting its importance in neuronal maintenance and integrity.