Abcam, ab265787, Human SQLE (Squalene Epoxidase) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SQLE KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon 1 and Insertion of the selection cassette in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SQLE, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Squalene epoxidase also called squalene monooxygenase is an essential enzyme in cholesterol biosynthesis. It converts squalene into 23-oxidosqualene through an epoxidation reaction involving the addition of an oxygen atom into the squalene molecule. The enzyme has an estimated mass of about 64 kDa and is primarily expressed in the liver where cholesterol synthesis is highly active. Its activity leads to the production of sterol intermediates which are further processed to form cholesterol and other sterols.
Biological function summary
Squalene epoxidase plays an important role in maintaining sterol homeostasis by regulating the conversion of squalene an important precursor in the cholesterol synthesis pathway. This enzyme operates as a part of the endoplasmic reticulum membrane complex where it anchors and acts on substrates to produce sterol intermediates for further enzymatic actions. Its function is tightly regulated by intracellular cholesterol levels to prevent overproduction and maintain cell membrane integrity.
Pathways
Squalene epoxidase is an important component of the mevalonate pathway a fundamental route for the synthesis of cholesterol and other isoprenoids. Besides cholesterol biosynthesis it connects to the synthesis of steroid hormones and bile acids. Farnesyl pyrophosphate another key intermediate in the mevalonate pathway influences the activity of squalene epoxidase. Moreover this enzyme cooperates with subsequent enzymes like lanosterol synthase to facilitate the continuation of sterol biosynthesis.
Dysregulation of squalene epoxidase can lead to conditions such as hypercholesterolemia which is associated with elevated cholesterol levels and cardiovascular disease. This enzyme's malfunction is also implicated in certain dermatological disorders due to disrupted sterol metabolism affecting skin barrier function. Alterations in squalene epoxidase activity can influence the expression and function of other proteins like HMG-CoA reductase which is tightly regulated by feedback mechanisms in response to intracellular cholesterol levels.