Abcam, ab265801, Human RHOT2 (MIRO2) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
RHOT2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 2 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 2 bp deletion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RHOT2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MIRO2 also known as Rhot2 or Mitochondrial Rho GTPase 2 is a mitochondrial Rho GTPase with a molecular weight of approximately 69 kDa. This protein belongs to the Rho family of GTPases and exists mainly on the mitochondrial outer membrane. It acts as a critical regulator of mitochondrial trafficking and dynamics. MIRO2 plays a pivotal role in the regulation of mitochondrial transport along the cytoskeleton by interacting with adaptor proteins. It features two EF-hand motifs and two GTPase domains that are essential for its function.
Biological function summary
MIRO2 contributes to the maintenance of mitochondrial network and quality within cells. It serves as a component of the calcium-sensing mitochondrial transport machinery and interacts with the transport protein complex including adaptor proteins like Milton and the motor protein kinesin. Through these interactions MIRO2 helps in coupling mitochondrial dynamics to cellular events by facilitating calcium-dependent docking and release of mitochondria. This function indicates the importance of MIRO2 in energy-demanding cellular processes.
Pathways
MIRO2 integrates into the mitochondrial transport and positioning pathways. It associates with the PINK1/Parkin pathway which is essential for mitochondrial quality control and turnover. The pathway leads to the balance of mitochondrial fission and fusion assisting in the removal of damaged mitochondria. Moreover MIRO2 collaborates with proteins like Mitofusins in these pathways to ensure efficient mitochondrial dynamics and bioenergetics.
MIRO2 has links to neurodegenerative diseases like Parkinson's disease. Dysfunction in the PINK1/Parkin pathway where MIRO2 participates leads to impaired mitophagy and neuronal cell death. Additionally imbalances in MIRO2 activity are implicated in the progression of certain types of cancer. In these contexts MIRO2 interacts with related proteins such as PINK1 and Mitofusins which play roles in disease mechanisms that involve mitochondrial dysfunction or aberrant cellular metabolism.