Abcam, ab265897, Human UBXN4 (Erasin) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
UBXN4 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 35 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 35 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-UBXN4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Erasin also known as X15H8 protein is a cellular protein with a mass of approximately 54 kDa. It functions as a molecular chaperone by helping in the folding and assembly of nascent polypeptides into functional proteins. Erasin is expressed mostly in liver and kidney tissues but can also be found in smaller amounts in other organs. This expression pattern supports its involvement in maintaining protein homeostasis and cellular proteostasis mechanisms in metabolically active tissues.
Biological function summary
The roles of Erasin extend beyond its chaperone activities. It is a component of a larger proteostasis network interacting with other cellular machineries to regulate degradation processes. Erasin helps in stabilizing misfolded proteins and prevents aggregation which could be harmful to the cell. Its presence in the endoplasmic reticulum signifies its function in protein quality control and stress response especially under conditions that challenge cellular homeostasis.
Pathways
Erasin actively participates in the unfolded protein response (UPR) and ER-associated degradation (ERAD) pathways. These pathways are important for eliminating defective proteins and maintaining cellular equilibrium. Erasin interacts closely with Bip and Calnexin both of which are key players in the ER stress response. By aiding these networks Erasin contributes to cellular health by ensuring that proteins achieve and maintain their correct structures.
Erasin engages in conditions related to protein misfolding and aggregation. Alzheimer's disease and cystic fibrosis are two such disorders where proteostasis is disrupted. In Alzheimer's disease the accumulation of misfolded amyloid-beta peptides links back to Erasin's role in protein folding and clearance. Similarly Erasin's interaction with DeltaF508-CFTR highlights its involvement in cystic fibrosis where proper folding of the CFTR protein is impaired. These associations highlight the pivotal role of Erasin in conditions that challenge cellular protein management.