Abcam, ab265950, Human LIX1L knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
LIX1L KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 5 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 5 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LIX1L, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LIX1L also known as Lim Homeobox Protein 1-Like is a protein encoded by the LIX1L gene with a mass of approximately 28.8 kDa. This protein is expressed in various tissues including the brain and testis indicating its role in multiple physiological contexts. LIX1L's structure suggests it could interact with DNA or proteins but its precise molecular mechanisms remain an area for further study.
Biological function summary
Lim Homeobox Protein 1-Like participates in processes linked to development and differentiation. It has been implicated in nervous system development possibly through interactions with transcriptional regulators. LIX1L may form part of larger protein complexes aligning with its expression in neural and reproductive tissues. Its presence in these tissues indicates roles in establishing cellular identity and tissue architecture.
Pathways
Lim Homeobox Protein 1-Like plays functions in developmental signaling pathways such as Wnt and Hedgehog. These pathways are critical for cell fate determination and tissue patterning. LIX1L might interact with proteins like beta-catenin in the Wnt signaling cascade and Gli in the Hedgehog pathway influencing their pathways and cellular outcomes.
LIX1L associates with conditions affecting the nervous system and reproductive organs. Neurological disorders like certain congenital brain malformations link with abnormal expression of LIX1L. Additionally reproductive issues possibly including infertility might arise from LIX1L's dysregulation. Studies suggest these associations through LIX1L's interactions with proteins such as GLI3 in the context of Hedgehog pathway dysregulation.