Abcam, ab266019, Human RRP36 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
RRP36 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon 1 and 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon 1 and 1 bp insertion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RRP36, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RRP36 also known as RRP36 homolog is a protein involved in ribosome biogenesis. It has a molecular mass of approximately 33 kDa. This protein is expressed chiefly in the nucleolus of eukaryotic cells. It plays an important mechanical role in the early stages of processing of precursor rRNA. RRP36 is essential for the maturation of the 90S pre-ribosome an important step in ribosome synthesis.
Biological function summary
The RRP36 protein functions as part of the small subunit (SSU) processome a large ribonucleoprotein complex. This complex plays a significant role in the early cleavage steps of pre-rRNA necessary for forming functional ribosomes. By contributing to pre-rRNA processing RRP36 aids in the efficient production of ribosomal RNA a core component of ribosomes. This precise processing is required for ribosome assembly and cellular protein synthesis.
Pathways
RRP36 integrates into the nucleolar small subunit processome-related pathway influential in ribosome biogenesis. This pathway involves many proteins including UTP4 and UTP15 which closely associate with RRP36 in the SSU processome. The successful operation of this pathway is critical for linking ribosome production to overall cellular growth proliferation and metabolism.
RRP36 shows a connection with disorders resulting from defects in ribosome biogenesis. Impaired ribosome production can contribute to ribosomopathies which include diseases such as Diamond-Blackfan anemia. In this context RRP36 operates alongside other ribosomal biogenesis-related proteins such as UTP18 emphasizing its role in normal cellular functioning and the consequences arising from its dysregulation.