Abcam, ab266079, Human SSBP4 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SSBP4 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 4

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SSBP4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SSBP4 also known as single-stranded DNA-binding protein 4 plays a role in DNA metabolic processes. It binds single-stranded DNA and assists in maintaining DNA stability during replication and repair. SSBP4 has an approximate molecular weight of 35 kDa. This protein is expressed in various tissues including the brain liver spleen and kidney contributing to its versatile functions in the human body.
Biological function summary
Single-stranded binding proteins including SSBP4 participate in managing genome stability by shielding DNA strands during replication. SSBP4 acts as a safeguard against DNA damage by preventing strand re-hybridization. Although SSBP4 operates independently its function is important in the complex machinery of DNA replication and repair.
Pathways
SSBP4 supports DNA replication and repair pathways often working alongside homologous proteins such as RPA (replication protein A). These pathways ensure accurate replication of the genome and help maintain genetic integrity during cell division. SSBP4's role in these pathways involves coordinating with other replication machinery to facilitate unwinding and protection of DNA strands.
Alterations in SSBP4 activity may be implicated in cancer particularly those involving genomic instability. Irregularities in DNA repair processes contribute to tumorigenesis making SSBP4 a potential focus for cancer research. Furthermore SSBP4 shares pathway connections with another involved protein BRCA2 which is also known for its role in maintaining DNA stability and is linked to hereditary breast and ovarian cancer.