Abcam, ab266100, Human TALDO1 (Transaldolase 1) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TALDO1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TALDO1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Transaldolase 1 also known as TALDO1 or TAL-H is an enzyme that plays an important role in the pentose phosphate pathway by catalyzing the transfer of a three-carbon dihydroxyacetone unit from sedoheptulose 7-phosphate to glyceraldehyde 3-phosphate producing erythrose 4-phosphate and fructose 6-phosphate. The molecular mass of Transaldolase 1 is approximately 37 kDa. It is expressed in various tissues within the body with higher expression levels observed in the liver and adipose tissues.
Biological function summary
Transaldolase 1 regulates the balance between the oxidative and non-oxidative branches of the pentose phosphate pathway essential for nucleotide biosynthesis and maintaining cellular redox status. It functions independently not as part of a larger enzyme complex. The activity of this enzyme affects cellular metabolic functions impacting the synthesis of nucleotides and pentose sugars which are necessary for cell growth and division.
Pathways
Transaldolase 1 is integral to the pentose phosphate pathway also known as the hexose monophosphate shunt. This pathway is important for providing ribose-5-phosphate for nucleotide synthesis and NADPH for reductive biosynthesis and cellular antioxidant systems. It links with glycolysis through shared intermediates such as fructose 6-phosphate connecting with enzymes like glucose-6-phosphate dehydrogenase. This relationship allows cells to adjust their metabolic flux according to physiological needs.
Transaldolase 1 mutations are associated with transaldolase deficiency a rare metabolic disorder that can lead to liver dysfunction and immunodeficiency. This deficiency affects the cellular ability to balance nucleotide and lipid biosynthesis contributing to the disease phenotype. There is also a connection with oxidative stress-related disorders as Transaldolase 1 indirectly influences reactive oxygen species via its role in NADPH production which ties it to proteins involved in oxidative stress responses like NADPH oxidase.